Antibacterial activity of silver nanoparticles of different. Antibacterial activity of zinc oxide and ag doped zinc. In this study, we describe the synthesis and characterization of silver nanoparticles agnps of different sizes and evaluated their antibacterial activity. These disks absorb the silver nanoparticles and become dry and hence there is no presence of chloroform.
Superior antibacterial activity of zinc oxidegraphene. Nanoparticle synthesis using plants is an alternative to conventional physical and chemical methods. Antibacterial activity of synthesized zno nanoparticles against s. Thus, zno in nanoscale has shown antimicrobial properties. In vitro evaluation of antibacterial properties of zinc oxide. Green synthesis of zinc oxide nanoparticles zno nps using ailanthus altissima fruit extracts and antibacterial activity. One of the main health problems is increasing resistance of bacteria to antimicrobial agents worldwide. However, chemical methods are more suitable for industrial scale production 35 due to its low efficiency and. The antibacterial activity of tadoped zno nanoparticles. In this paper, we report the synthesis of zno nanoparticles using moringa oleifera drumstick leaves as natural precursor via precipitation method. This study investigates the antibacterial activity aba of suspensions of pure zno nanoparticles znonps and mesoporous silica doped with zno znouvm7, as well as electrospun nanofibers containing those nanoparticles.
In the presence of four pathogens such as staphylococcus aureus, escherichia coli, salmonella typhimurium, and. Zinc oxide zno is an inorganic compound widely used in everyday applications. Antibacterial activity of polypropylene zno nanoparticles. Pdf antibacterial activity of zno nanoparticle on gram. Therefore, we aimed in this study to test the antibacterial activity of zno nanoparticles on important and perilous bacteria such as p.
New materials with good antibacterial activity and less toxicity to other species attract numerous research interest. Biosynthesis of zinc oxide nanoparticles and assay of antibacterial activity 1emad j. To further evaluate the released zn ion antibacterial activity, the density of s. A novel photocatalyst of tadoped zno nanoparticles was prepared by a modified pechinitype method. However, chemical methods are more suitable for industrial scale. Green synthesis of zinc oxide nanoparticles znonps using ailanthus altissima fruit extracts and antibacterial activity. Zno nanoparticles against pathogenic bacteria and fungi.
Badawy 1sulaimani directorate of health, ministry of health kurdistan, iraq 2department food science, college of agriculture, tikrit university, iraq article history received. Antibacterial activity of zinc oxide nanoparticles znonps has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region. Metal nps are among the most promising of these because show strong antibacterial activity. The typical bacterium escherichia coli and hela cell were used to evaluate the antibacterial activity and cytotoxicity of the zno go composites, respectively. Here, we show that small zinc oxide nanoparticles zno npspyramids, plates, and spherespossess the ability to inhibit activity of a typical enzyme. The antimicrobial study of tadoped zno nanoparticles on several bacteria of grampositive bacillus subtilis b.
Green synthesis of zinc oxide nanoparticles znonps using. Zno nanoparticles degraded almost 96% of titan yellow dye. Apr 19, 2015 antibacterial activity of zinc oxide nanoparticles zno nps has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region. Zinc oxide zno is a provocative material for applications in electronics, photonics, acoustics and sensing. The antibacterial effect of zinc oxide zno nanoparticles oncampylobacter jejuni was investigated for inhibition and inactivation of cell growth. Zno nanoparticles can be obtained by physical and chemical routes. Synthesis and characterization of zno nanoparticles using.
Antibacterial activity of zno nanoparticle suspensions on a. Antibacterial activity of zno nanoparticle on grampositive and gramnegative bacteria article pdf available in african journal of microbiology research 518 june 2012 with 9,983 reads. Green synthesis, characterization and antibacterial. Nanoparticles with antibacterial element incorporation into. As the field of nanomedicine emerges, there is a lag in research surrounding the topic of nanoparticle np toxicity, particularly concerned with mechanisms of action. To investigate antibacterial activities of zinc oxide nanoparticles zno np and their mode of action against an important foodborne pathogen, escherichia coli o157. Zno nanoparticles induced significant morphological changes, measurable membrane leakage, and substantial increases up to 52fold in oxidative stress gene expression in c. Zno is currently listed as a generally recognized as safe gras material by the food and drug administration and is used as food additive. Antibacterial activity and mechanism of zno nanoparticles. In addition, zno nanoparticles zno nps exhibit antibacterial activity and can reduce the attachment and viability of microbes on biomedical surfaces brayner et al. It is commonly reported that znonps antimicrobial activity is associated w national nanotechnology day celebrating our 2018 prize and award winners. Growth kinetics and disk diffusion assay showed that znonp demonstrated good antibacterial activity against carbapenem resistant a. Many microorganisms exist in the range from hundreds of nanometers to tens of micrometers. Nanosized particles of zno have more pronounced antimicrobial activities than largesize particles due to small size and high surfacetovolume ratio.
The present study of antibacterial activity of zno npi and zno npii against staphylococcus aureus suggest that both zno npi and zno npii have a little retarded antibacterial effect against s. In this work, the effect of zinc oxide zno nanoparticles prepared by mechanochemical method on the antibacterial activity of different antibiotics was evaluated using disk diffusion method against staphylococcus aureus and escherichia coli. Initial cell concentration was around 109 cfu ml1, and aqueous znonps suspensions ranged. Properties of zinc oxide nanoparticles and their activity. Feb 04, 2011 in summary, zno nanoparticles exhibited remarkable antibacterial activity and demonstrated a lethal effect against c. Antibacterial activity of zno nanoparticles with a. Pdf antimicrobial activity of zno nanoparticles against. When the size is 12 nm, it inhibits the growth of s. But the ag doped zinc oxide nanoparticles showed better antibacterial activity than the zno nanoparticles. Pdf the antibacterial activity of tadoped zno nanoparticles. Y h leung 1, c m n chan 2, a m c ng 1,3, h t chan 4, m w l chiang 5, a b djurisic 1, y h ng 1, w y jim 1, m y guo 1, f c c leung 2, w k chan 4 and d t w au 5.
Antibacterial activity of zno nanoparticles prepared via non. The antibacterial activity of zno is dependent on the size and concentration of the particles. All tnts with zno nanoparticles showed significantly low bacteria density in the supernatant compared with both polished ti and tnts. The disc diffusion method for antibiotic susceptibility testing is the kirbybauer method. Antibacterial activity and mechanism of action of zinc oxide. Published 26 october 2012 2012 iop publishing ltd nanotechnology, volume 23, number 47. The antibacterial activity of zno may be dependent on the size and the presence of normal visible light. Biosynthesis of zinc oxide nanoparticles and assay of.
Therefore, we aimed in this study to test the antibacterial activity of zno nanoparticles on important and perilous bacteria such as. In the composites, zno nanoparticles nps, with a size of about 4 nm, homogeneously anchored onto go sheets. The continuous emergence of bacterial resistance has challenged the research community to develop novel antibiotic agents. Original article antibacterial activities of zinc oxide. Mechanism of antibacterial activity of zinc oxide nanoparticle. Pdf antibacterial activity of zinc oxide nanoparticles on. However, it is not yet clear what change in antibacterial activity is expected by changing the lattice constant of silver nanoparticles. It is known that the biological synthesis of nanoparticles is gaining importance due to its simplicity, ecofriendliness and exten. Pdf antibacterial activity of zinc oxide nanoparticles znonps has received significant interest worldwide particularly by the implementation of. In both the methods zno nanoparticles with 100microgl showed best antibacterial activity, and further studies on destruction of bacterial genomic dna was done using pcr and gel electrophoresis revealed the dna fragment bands, this activity might be due to surface charge. Stock cultures were prepared by inoculating the strains in tryptone soya agar tsa himedia, india, followed by.
The advent of nanotechnology has led the development of materials with new properties for use as antimicrobial agents. Zno nanoparticles have significantly higher antibacterial effect on s. The use of zinc oxide nanoparticles zno nps to optimise the fight against microbial resistance has been receiving increased attention due to the nonspecific activity of inorganic antimicrobial. Pdf the aim of the present study is to determine the antimicrobial activity of zno nanoparticles against gramnegative and grampositive bacteria find. In the present study the antibacterial activity of zinc oxide zno nanoparticles was investigated against gram negative escherichia. Evaluation of the bacteriostatic effects was performed by ultravioletvisible spectrophotometry and comet assays. We also studied the antibacterial activity and it was found that the synthesized zno nanoparticles. Antibacterial activity of modified zinc oxide nanoparticles.
Both nanozinc oxide and bulk zinc oxide are 4080fold less toxic than znso 4 against v. Pdf antibacterial activity of zinc oxide nanoparticles. Aug 21, 2015 a novel photocatalyst of tadoped zno nanoparticles was prepared by a modified pechinitype method. In the case of the nanoparticles with smaller particle sizes, only 3050% of the antibacterial activity is retained at 5 mm concentration up to. Venkatesha5, s t arun kumar6 1 department of chemistry, ssmrv degree college, jayanagar 4th t block, bangalore. Superior antibacterial activity of zinc oxidegraphene oxide.
Antibacterial activity of zinc oxidenanoparticles znonps has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region. Compared with the irregular zno nanoparticles, the nanorod zno particles on the tnts surface showed easier penetration into bacteria membrane, and improved the antibacterial properties. The antibacterial effect of zinc oxide zno nanoparticles on campylobacter jejuni was investigated for inhibition and inactivation of cell growth. Nanoparticles zinc oxide antibacterial antifungal reactive oxygen species. Zno nanoparticles enhanced antibacterial activity of. A study on the antibacterial activity of zno nanoparticles. Titan yellow under visible light by exposing the visible light for one hour. Biosynthesis and antibacterial activity of zno nanoparticles. Nanoparticles were prepared via nonhydrolytic solution process using zinc acetate dihydrate znch 3 coo 2 2h 2 o and aniline c 6 h 5 nh 2 in 6 h refluxing at. Preparation and antibacterial activity of silver nanoparticles. The synthesized zno nanoparticles have been employed as photocatalytic agent to degrade the organic dye viz. Antibacterial activity and mechanism of action of zinc. The antibacterial activity of tadoped zno nanoparticles article pdf available in nanoscale research letters 101.
Research article antibacterial activity of agdoped tio 2 and agdoped zno nanoparticles gebretinsae yeabyo nigussie,1 gebrekidan mebrahtu tesfamariam,1 berhanu menasbo tegegne,1 yemane araya weldemichel,1 tesfakiros woldu gebreab,2 desta gebremedhin gebrehiwot,1 and gebru equar gebremichel3 1department of chemistry, college of natural and computational sciences, mekelle. Antibacterial activity of agdoped tio and agdoped zno. Apr, 2016 in vitro antibacterial activity of zno and nd doped zno nanoparticles against esbl producing escherichia coli and klebsiella pneumoniae. The antibacterial activity increases with increasing zno nanoparticles concentration. Zinc oxide nano particles are better antibacterial agent 2122. Antimicrobial activity of zno nanoparticles against. In addition, zno nanoparticles znonps exhibit antibacterial activity and can reduce the attachment and viability of microbes on biomedical surfaces brayner et al. Particles size and morphology were characterized by transmission electron microscopy. In vitro antibacterial activity of zno and nd doped zno. The results indicated that zno nanoparticles synthesized from t. Zno np with sizes of 70 nm and concentrations of 0, 3, 6 and 12 mmol l1 and npfree solutions were used in antimicrobial tests against e. The average size of zno nanoparticles was between 20 nm and 45 nm. The data suggest that zno nanoparticles have a potential application as a bacteriostatic agent in visible light and may have future applications in the development of derivative agents to control the spread and infection of a variety of.
Taking advantage of zinc oxide zno and graphene oxide go, the znogo composites were prepared by a facile onepot reaction to achieve superior antibacterial properties without damaging other species. Pdf characterization and antibacterial activity of zno. Antibacterial activity of zno nanoparticles with a modified surface under ambient illumination. The mechanisms of antibacterial activity of zno particles are not well understood, although some statements were proposed such as, generation of hydrogen peroxide could be the main factor of antibacterial activity xie et al. Antibacterial activity and inflammation inhibition of zno. Green synthesis, characterization and antibacterial activity. Enzyme inhibitors are ubiquitous in all living systems, and their biological inhibitory activity is strongly dependent on their molecular shape. Antibacterial activity and mechanism of zno nanoparticles on. Antibacterial activity of zno nanoparticles prepared via. Pdf antibacterial activity of zno nanoparticle on grampositive. The antibacterial activity of zno nanoparticles was evaluated by measuring the zone of inhibition against the test organisms.
Increased antibacterial activity of zno nanoparticles. The antibacterial activity of znonps was tested against grampositive bacteria, bacillus cereus atcc 11778 and staphylococcus aureus atcc 25923, and gramnegative bacteria, pseudomonas aeruginosa atcc 27853 and salmonella typhimurium atcc 1428. Zinc oxide zno nanoparticles have received a remarkable consideration due to their extra ordinary uv filtering properties, photochemical antifungal, high catalytic and antibacterial activities. The overall percentage reduction of li also increased in higher catalyst concentration 0. The minimum inhibitory concentration mic and minimum bactericidal concentration mbc of these two materials were also determined under the same conditions. In this study, the antimicrobial activity of titanium dioxide tio2, zinc oxide zno, and tio2zno nanoparticles supported into 4a zeolite 4a z was. The sizes of the zones of growth inhibition are presented in table 1. Antibacterial activity of polypropylene zno nanoparticles fibers. A study on the antibacterial activity of zno nanoparticles prepared by combustion method against e coli durga prasad1, c. Unexpected insights into antibacterial activity of zinc. In the composites, zno nanoparticles nps, with a size of about 4. Antibacterial activity abstract zinc oxide zno has broad applications in various areas. Antimicrobial activity of titanium dioxide and zinc oxide.
Combined effect of ultrasound stimulations and autoclaving. Nanosized particles of zno have more pronounced antimicrobial activities than largesize particles. Zinc oxide nanoparticles znonps are attractive as broadspectrum antibiotics, however, their further engineering as antimicrobial agents and clinical translation is impeded by controversial data about their mechanism of activity. Formation and characterization of zno nanoparticles was established by uvvis spectroscopy, fourier. The antibacterial activity of zno nanoparticles has been investigated and presented in this paper. Pdf increased antibacterial activity of zno nanoparticles. Antibacterial activity of zinc oxide and ag doped zinc oxide. Synthesis,characterization and antibacterial properties of zinc oxide nanoparticles 47 particle size, morphology and structure 3537. Pdf antimicrobial growth inhibition and mechanistic activities of synthesized zinc oxide nanoparticles zno nps were investigated in this study find, read.
907 1287 671 678 343 770 725 37 1501 894 16 1159 240 673 949 1010 903 803 876 145 145 520 1036 1427 4 1051 794 75 167 1499 1157 697 862 819 194 881 1354 761 623 1364 839 1153